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Tried stopping your antivirus (a/v) if you are running one?
I know it sounds odd, but I have seen it happen with some external usb hdd's when the a/v playing up would do background scans preventing a dismount/eject of the drive. Some a/v can even have exceptions setup to not scan certain removable devices if you so require to prevent such issues or other performance impacts. Norton a/v etc.. was particularly bad at this sort of thing in the past.
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