One other thing you might consider is to check if any recent driver updates have occurred, especially for your mouse or touchpad. This could potentially affect drag & drop functionality.
Also, even though you've looked at permissions, it may be worth double-checking that your user account has full control permissions for the Calibre directory and files.
Lastly, you might want to see if there's an issue with the specific file types you're trying to drag and drop. Are all types of books not working, or just specific formats?
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