I have become averse to light mode on backlit devices, so use dark on my phone, tablet and PC monitor, which obviously includes Calibre. The bigger the screen, the more the bright hurts, so as monitors went from 24" to 27" to my current 34", my preference for dark mode grew. Ditto the jump from an 8-inch tablet to an 11-inch. For actual reading, the reverse is true and dark mode has never been spotted on any of my e-ink devices.
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