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Kindlegen produces a Master MOBI, which includes both the older MOBI7 and newer KF8 formats in one file. If you sideload that file those Kindle apps and devices that support KF8 will use the KF8 part and the rest (mainly older Kindles and the Kindle for iOS app) will use the MOBI7 part.
When you publish a book on Amazon the publishing process produces additional formats, usually KCR and KFX. KFX, the format behind Enhanced Typesetting, is sent to most newer apps and devices. KCR is sent to the Kindle Cloud Reader and sometimes to Kindle for iOS.
Amazon does not provide a method to produce your own KFX files for testing. Instead the Kindle Previewer produces a variant of KFX (called KPF) and uses that to show how the book will look on newer Kindle apps and devices. As an alternative I have developed the KFX Output calibre plugin to allow the generation of KFX files for personal use and testing, however the Kindle Previewer should be sufficient for most purposes.
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