You should not need an Advanced rule
You need a column to indicate you've read the book, you could use Rating - something like the following (whilst Rating shows as stars internally its a number between 0 and 5). This colours the line
Or you could create a Y/N custom column and do the following. This colours the Title Cell only.
But IMO if you have a Y//N column you don't need to colour anything - Y/N columns are displayed as a Green Tick/Red Cross glyph.
I think some e-readers can return a 'read' status to calibre, as can Calibre Companion
BR