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A line, 800x1 pixels long, will be displayed exactly at 800 pixels wide, 1 pixels high, on an 800x600 screen. ("Created for an 800 pixels wide screen.") If you show that same line at 100%, on an e-reader with a 1600x1200 screen, it will reach only up to half of the screen, and it'll be half as thick.
Because the screen has double the pixels, the line will need to be scaled up to 1600x2, and thus the reader will need to 'invent' every other pixel, as it's not there. In case of a line this will not be a problem, but an image will become blurry.
If the resolution is not exactly x2 on each side, scaling will be worse. (Nowadays, algorithms are good enough to also handle 1.5x or 2.5x, for example, but if it's something like 2.3, or 1.75, your image will definitely be blurry.)
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