The usual reason (90% or so) for this to happen is the antivirus on the calibre machine deciding that CC is evil. Antivirus apps update themselves sometimes multiple times per day. The fix is to tell (possibly yet again) the AV to allow connections to calibre.
The second much more rare reason is that the home router restarted and "forgot" information about the devices. Rebooting both the device and the calibre computer usually fixes this.
If you remain stuck after looking that above two, send me a CC debug log. That will tell me what CC is seeing. Sending (or posting) a calibre debug log could be helpful as well, as that would tell us if calibre is seeing anything at all from CC.
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