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No. *.pyo optimized bytecode is the cached version of what the python interpreter would have translated the *.py source code into, during the process of running. When *.pyo files exist the *.py files are actually ignored entirely.
Distributing an application as *.pyo files is pretty standard for compiled binary applications. It speeds it up, saves space, and even provides rudimentary protection for closed-source binaries.
The calibre binary download has always distributed *.pyo files.
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