Let try a recap. I used to be able to simply connect my Kindle unit to my desktop computer via USB cable ; Calibre would automatically acknowledge the unit and titles previously downloaded to the unit would appeared as "On Device". Not anymore : I seems only able to connect via the wireless option but even that is unstable : if the unit is left unattended for a while, the "On Device" listing disappear from the Calibre interface and yet the Kindle continue to indicate connected. I must manually disconnect then reactivate the wireless option. As suggested, I have disable my anti-virus to check if the problem was there but it did not change anything.
Also titles sent to my Kindle after 29-Nov-15 are not listed as "On Device" although they are sitting in my Kindle. The only major change around then was when I upgraded Calibre to 2.45.0
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