Same issue here on a machine upgraded from Windows 7.
While I appreciate David's advice to do a clean install of Windows 10, I don't feel like going through a reinstall of dozens of programs and settings just to fix a behavior I am only seeing in Calibre. I find it odd that other programs that access external USB devices (like my GPS, iPhones, etc.) have zeros issues. There must be some difference in the way Calibre detects Kindles or Kindles behave at some OS-related level.
For know, if going to trying running Calibre in a Windows 7 VM and see if that gives me any joy. If that fails, maybe I just use Calibre as a pretty e-book catalog.
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