Also, Windows 10 is a new OS and doesn't "know" the devices that the previous OS knew. The first time a USB device is plugged in Win10 has to do its "touchy feely who-are-you" negotiations with the device. Only when these are finished is all device interaction available to apps.
You should first introduce the device to Win10 without any apps that interact directly with the device (e.g. calibre, ...) running. After that first "introduction" session, you should be able to return to your normal procedure.
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