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There's no reason whatever to do anything with that epub other than upload it to the KDP. In my experience, epubs convert beautifully.
If you want the Amazon "mobi" (it actually contains multiple books files), you can download that from the KDP at Step 7 in the publishing process. Indeed, I recommend doing just that, as the best way to check the book in the (downloaded) Kindle Previewer.
I would keep Calibre out of it altogether. Calibre-generated mobis evidently no longer are routinely rejected by the KDP, but apparently it does still sometimes happen.
Sigil rules! See my thread with that title.
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