[eschwartz]

Theoretically yes, but it is discouraged:

Originally Posted by ixtab

Make sure that you are performing steps 3 and 4 with physical hardware, i.e., on a real computer, and with a real CD/DVD or USB stick, not in a virtual machine!

Spoiler:

Yes, I do know that it also works in a Virtual Machine, provided that you make sure the device (which enters 3 different modes) is actually seen by the VM in all of these modes. For the average end user, the VM simply constitutes another layer of potential trouble - which is why I don't suggest to run Kubrick in a VM.

You should be able to disable secure boot, and hopefully simulate BIOS mode -- that will break booting into Windows, but allow booting old-style systems and you can then switch your UEFI settings back when you are done.