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Ricky's suggestion on just Uninstalling the drivers from the System and restarting the system to allow them to be reinstalled is spot on, as I've used that method in the past to fix issues not just with USB, but with other devices as well.
Re: SP3
I've not seen any issues since installing SP3, so you should probably not run into any major problems.
Still, usually I tend to reinstall my OS once or twice a year anyway, and install Major updates at that time on a clean build before putting my other apps onto the system.
Less problems that way.
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