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I am not familiar with VirtualBox (I have used others though), but if you are using a removable drive as mentioned and it is USB, then my first investigation would be checking VirtualBox's USB support. It may be just that it has to be enabled, or it has to be explicitly checked to see if it is still attached in each session, or there may be some trickery required according to what host OS you are using and which Oracle's VirtualBox technical support documentation may assist in.
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