At some point you are going to need access to an eink-reader. Some of their behavior and formatting features are strange. Keep in mind that the same Adobe Adept ebook software is built into Nook, Sony, kobo etc
I'll be buying a Nook touch precisely for testing (will receive Thursday)
The barnes and noble desktop ebook reader doesn't really simulate how an ebook really looks. you might as well be testing with Adobe Digital Editions.
Here are some things which jumped out at me about the Nook Touch
TOC only support one level
they have a user setting to enable publisher defaults.
hyphenation for titles is semi-broken. See this fix
http://www.pigsgourdsandwikis.com/20...ook-color.html (I suspect it involves both nook color and touch)
widows and orphans seem to work ok. See (Liz Castro's Epub Straight to the Point ebook for details).
These are thoughts off the top of head.
Oops UPdate: It looks like Nook Color and Touch are not based on Adobe SDK, but Nook can apparently display ebooks through Adobe
https://www.mobileread.com/forums/arc.../t-107697.html
So you can probably expect slight differences between it and Sony Reader for example.