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If the supplied font is in the right format and embedded correctly then yes. It depends on the implementation of the reader of course what the correct method of embedding is and whether it supports it at all.
However, if your reader has enough memory you could use as much fonts as you would like (separate from legal issues of course). I doubt that reading the book is pleasurable or fast though. Since the font is embedded in the epub, it will only be read/used when the book is openend. I think it will open the font only when used, but I am not sure.
If you specify a font family or type which the reader does not have and is not included in the epub, the reader will decide. Usually readers will use 2 fonts for that, serif and non-serif. However, the epub must still validate of course.
The font size depends on the reader. Some have only 3 sizes (like my PRS-300). That is the reason why a lot of epub-creators use em as size measure instead of pt. In this case 1 em is the default font size of the reader. In the PRS-300 case that is the setting on S and the letters are about 3-4 mm I think. It could happen that if you specify the font in pt or px that sizing the font will no longer work. Could be nice for chapter headings (stress could), but in general I think that is a pain in the a*s.
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