The memory card is not necessary. Calibre works great for me with no special work.
It seems obvious but it is worth mentioning: the Nook Color won't mount while it is asleep (although it will remain mounted if it returns to sleep). After you plug it in, you have to press power, unlock, and you'll see a message on the display indicating that you are now connected to the computer.
Wait a few seconds for Calibre to recognize the device.
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