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You can create multiple subject entries (tags) if you want. But as the Wolf says, it doesn't really have anything to do internet search engines. Most ebook stores don't even have the ability to allow you to search an ePub's subject tags.
Just know that most people who have large ebook libraries are often very picky about the tags they use in calibre and such. So if you include subject metadata, you'd probably be wise to keep it to very few and to keep them very general. The people that know about those subject tags are going to change them to their liking anyway. The people that don't know about them will never see them. *shrugs*
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