Originally Posted by kiwidude
??? What do you mean? As I understand it this file is only used for displaying a cover in iTunes. Either you are interested in putting your ePubs in iTunes (in which case you won't bother removing any iTunes files), or you aren't (in which case removing any seems perfectly safe and sensible). There is no code in Calibre I could find that looks for this filename.
The file is an image file. So if the cover in the epub is set to use this file, your cover wil be broken (not a real problem if it is allready set in calibre, but you will loose your option to set the cover from the file.
So to use this save, you should be sure the file is not manifested, but also not directly linked from one of the (x)html files